Empirical midpoint dissociation temperature (Td) determination for oligonucleotide probes using a PCR thermal cycler
Publication information:
Gulledge, and Cavanaugh. (October) 1999. “Empirical Midpoint Dissociation Temperature (Td) Determination for Oligonucleotide Probes Using a PCR Thermal Cycler”. Biotechniques 27: 666-8, 670.